FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786413
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33450.18724673409788217No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18600.10411926021586275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC38300.037.3903962
GTGGTAT39000.036.732651
GTATCAA132150.025.5303381
AGAGTAC86150.024.19725411
CAGAGTA88500.023.73984710
TATCAAC145100.023.148612
ACCGGTC600.002040083322.9087098
TAACGGC1757.2759576E-1222.00283436
GGTATCA68850.021.7952163
ATCAACG157050.021.3323273
TCAACGC157050.021.2623274
AACGCAG157350.021.15376
CGCGGTA1851.8189894E-1120.80824743
GCGGTAC1851.8189894E-1120.80824744
CAACGCA160800.020.7835645
AGTACGG100250.020.49219113
AACGGCC1902.5465852E-1120.2657737
GAGTACG103150.020.07603312
ACGGGAT13450.019.62913116
CGGGATC5200.019.56763617
Produced by FastQC (version 0.11.5)