FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917610
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17660.19245649023005418No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG11130.12129335992415077No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT9400.10244003443728818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20750.043.4591521
TGGTATC20650.043.25842
GTATCAA76600.026.129321
GGTATCA40000.023.8503631
AGAGTAC49750.023.82733311
TATCAAC84650.023.5406362
CAGAGTA51250.023.29094310
ATCAACG92600.021.4282153
TCAACGC93100.021.2836154
CAACGCA94750.020.883975
AACGCAG96850.020.4890146
AGTACGG58150.020.33808313
GAGTACG59150.020.04074312
ACGGGAT13450.019.62982716
AGTGGTA4550.019.3306182
CGGGATA2850.018.33480617
CGCAGAG109500.018.19938
GTACGGG64500.018.1652414
ACGCAGA109350.018.1478657
TACGGGA32900.018.05714615
Produced by FastQC (version 0.11.5)