FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689701
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.3921995666688959No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.30431419523335784No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38500.22785096298102445No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.16618324780538093No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26750.15831203272058192No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.11824577247690568No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.1004911519848778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25600.055.1451381
TGGTATC26250.053.4271932
GTATCAA103050.041.5931361
TATCAAC114850.038.002942
GGTATCA50750.035.7767561
TCAACGC122150.035.6274074
ATCAACG122850.035.4593
CAACGCA125500.034.7779855
AACGCAG128300.034.0852476
ACGCAGA144550.030.224957
CGCAGAG145800.029.965828
GCAGAGT152850.028.6949129
GTCGAGG18650.024.8392358
CAGAGTA76200.024.48500310-11
TCGAGGT18750.024.026769
AGAGTAC72750.022.49149710-11
ATACGGG1754.3269756E-721.8682291
GAGTACG89150.020.73760212-13
AGTCGAG26450.020.7278677
GACAGGT10350.018.8984661
Produced by FastQC (version 0.11.5)