FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258286
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56490.44894403974930974No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.2911102881220963No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.1994777022076062No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA23160.18405990371028524No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC17250.1370912495251477No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT17220.13685282996075612No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG15010.11928925538391114No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14070.1118187756996422No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC13300.10569934021359213No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13090.1040304032628512No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA13010.10339461775780705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16050.050.8400542
GTGGTAT17250.047.5610661
GTATCAA60000.041.446511
TATCAAC69300.036.2438282
GGTATCA30050.035.506891
ATCAACG71300.035.1661643
TCAACGC71450.035.1518174
CAACGCA72600.034.536475
AACGCAG74050.033.9749766
ACGCAGA80800.031.0828917
CGCAGAG81300.030.8394588
GCAGAGT88250.028.4588959
CAGAGTA54200.025.3641510-11
AGAGTAC51050.023.3913810-11
CGTCAGA2603.6379788E-1222.8832265
GAGTACG60050.022.61013212-13
AGCGTCA2801.2732926E-1121.2487073
GTACTGG6650.020.4555761
AGTACGG58400.020.37466412-13
GCGTCAG3003.45608E-1119.8321294
Produced by FastQC (version 0.11.5)