FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258286
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18920.1503632719429446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17240.1370117763370172No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC15840.12588552999874433No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT15170.12056082639399945No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT12980.1031561981934155No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12670.10069252936136935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54300.030.6989771
GTGGTAT10800.029.545091
TGGTATC10850.029.143822
GGTATCA24650.027.0053331
TATCAAC63100.026.5378862
ATCAACG64950.025.6901743
TCAACGC65450.025.4929034
CAACGCA66200.025.3721925
AACGCAG65850.025.3420776
CAGAGTA61400.025.30120310
AGAGTAC60950.025.21934111
ACGCAGA68150.024.4061267
CGCAGAG68450.024.1786678
GCAGAGT72900.022.6300149
ACGGGTA3050.022.54264616
TGCCGAC4150.022.53357355
AGTACGG67650.022.31871813
GAGTACG69400.021.99019812
CGGGATT3000.021.08488817
TACGGGT13400.020.72914115
Produced by FastQC (version 0.11.5)