FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912101
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.37627411876535605No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.35335998973797855No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.22354980424317042No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.1833130322190196No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.14066424661303956No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.12290305569229723No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA10450.11457064513688724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15800.055.948392
GTGGTAT16350.054.1048931
GTATCAA55500.037.701891
GGTATCA31000.035.669961
TATCAAC61000.034.278092
TCAACGC66050.031.785974
ATCAACG66550.031.4832973
CAACGCA67800.030.9655385
AACGCAG69450.030.2298556
GTCGAGG8400.029.3447288
CGCAGAG75200.027.9749138
ACGCAGA76350.027.4978877
GCAGAGT79300.026.4749479
TCGAGGT9250.026.1887349
CAGAGTA42350.023.98489210-11
GACAGGT4100.022.8207451
CGTCAGA2104.5802153E-922.2615185
GCAGCGT2305.366019E-1022.189341
AGTCGAG13600.021.5621247
GAGTACG47650.020.9609112-13
Produced by FastQC (version 0.11.5)