FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912101
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17910.19635983295709578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17800.042.4672362
GTGGTAT18450.041.4316861
GTATCAA63100.025.8410111
AGAGTAC40550.024.68958711
CAGAGTA41700.024.20657210
GGTATCA33100.023.5821843
TATCAAC70550.023.0656642
GAGTACG46800.021.33359712
AGTACGG47000.021.18429413
TCAACGC77250.020.9193744
ATCAACG77200.020.8628673
CAACGCA78600.020.6300055
AACGCAG80300.020.2981596
CGATCGT700.00496427819.65036247
GCGCTCG700.00497058319.6460511
TCGTATA700.00498637519.63527932
ACTCCGG700.00498953919.6331256
TTCGTAT856.7023066E-419.40321231
ACGGGAC3900.019.04155516
GTACGGG52650.018.75423614
Produced by FastQC (version 0.11.5)