FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767264
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.416745885164865No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.297352291451645No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.2173981929128868No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT21130.11956334763793072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19200.051.3535352
GTGGTAT19800.050.02051
GTATCAA67600.039.048311
GGTATCA36400.036.317531
TATCAAC76000.034.838392
ATCAACG79650.033.133313
TCAACGC79950.032.955834
CAACGCA81150.032.46855
AACGCAG84850.031.1027476
ACGCAGA93400.028.2092517
CGCAGAG94000.027.983988
GCAGAGT98700.026.7813369
CAGAGTA56550.024.499110-11
AGAGTAC53450.021.54699510-11
GAGTACG65800.020.9907912-13
GTACGGG71300.019.28217714-15
AGTACGG60450.019.0170112-13
GTCTAAC2051.9818344E-618.6614761
AGCGTCA4400.018.3510073
CGTCAGA4550.017.7460295
Produced by FastQC (version 0.11.5)