FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767264
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48380.27375649591685225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13100.036.094572
GTGGTAT14050.033.865971
GTATCAA58350.028.5644251
GGTATCA28700.025.8747181
TATCAAC67350.024.8578992
AGAGTAC62950.023.80383711
ATCAACG69650.023.7961773
TCAACGC69700.023.7784334
CAACGCA71350.023.3467545
CAGAGTA65750.022.83130610
AACGCAG73250.022.742466
GTACGAC1757.2759576E-1221.9887733
CGCAGAG75650.021.9852398
ACGCAGA76300.021.8693857
AGTACGG70600.020.76189813
GCAGAGT81300.020.527319
GAGTACG73950.020.11716312
TACGGGG33150.019.16450315
GTACGGG78900.018.474314
TGTACCG1352.3576795E-618.3255335
Produced by FastQC (version 0.11.5)