FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246673
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61590.4940349233519937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8350.033.2815671
TGGTATC9000.030.8555952
GTATCAA41300.029.9799791
GGTATCA19550.028.4297771
TATCAAC47450.026.191312
TCAACGC49350.025.003754
CAACGCA49850.024.9734765
ATCAACG49550.024.8503533
AGAGTAC46250.024.31457311
AACGCAG51400.024.0648146
CAGAGTA48200.023.55906510
CGCAGAG52450.023.532548
ACGCAGA53100.023.2426137
AGTACGG50950.021.8044213
GCAGAGT57350.021.5715759
GCTATAC650.003250060421.142433
GAGTACG53950.020.74235312
ACGGGTA1601.3387762E-920.6254516
TCGACAA956.059102E-520.26360118
TTAACGG1108.233443E-620.00525735
Produced by FastQC (version 0.11.5)