FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675251
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24840.3678632093843623No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.32447193710190725No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.2185853852863602No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.1996294711151853No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.14379838015789684No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.12795242065543036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14400.052.5494651
TGGTATC14700.052.329112
TGTGCGC2350.039.786618
GTGCGCT2550.036.6660929
TTGTGCG2350.036.1696477
GTATCAA50000.035.795041
GGTATCA28300.035.0009881
TATCAAC56350.032.4307252
TCAACGC60100.030.265754
ATCAACG60550.030.0408153
AACGCAG61000.029.7495336
CAACGCA61400.029.6941595
GCTTGTA3150.026.99171
ACGCAGA67850.026.6208067
CGCAGAG69600.025.951468
CGTCAGA2009.458745E-1125.4996015
GTCGAGG9200.024.9452618
GCAGAGT72900.024.7767059
GCAGCGT1901.4333636E-924.6121671
ACGGGTC2800.024.2853345
Produced by FastQC (version 0.11.5)