FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713529
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.3236028248326277No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.30454263246483326No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.19999187138854904No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.17995063970770636No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.13089867405529418No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.11408085726018144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14600.047.4483952
GTGGTAT15150.044.903151
GTATCAA46200.036.0755961
TATCAAC53300.032.054252
TCAACGC57250.029.991124
ATCAACG57350.029.9388243
GGTATCA27150.029.9111391
AACGCAG57200.029.8687346
CAACGCA58300.029.6696645
GCTTGTA2600.029.4353071
GTCGAGG8050.027.453288
CGCAGAG65600.026.3032238
ACGCAGA65550.026.2584537
GCAGAGT68500.025.0655699
TCGAGGT9650.022.461039
ACGGGCC1903.8729922E-822.368315
CAGAGTA37550.022.29682710-11
ACGGGTC2058.650568E-820.7316065
AGTCGAG13450.020.5389337
AGACCGT1254.7811295E-420.39996
Produced by FastQC (version 0.11.5)