FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713529
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11720.16425401069893444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG257.868879E-443.983639
GTGGTAT17700.038.232911
TGGTATC18000.037.4192472
CTGTCGC651.09115244E-425.3751689
GCCGTTA550.001224749825.00471915
CCGTTAA550.001224749825.00471916
GCTTGTA1900.023.1655141
CACGGGC600.002035863222.91456442
GTATCAA54850.022.6692621
GGTATCA32850.021.6890221
CAGAGTA39700.021.61465510
AGAGTAC39000.021.57948111
GGCCGTT650.00323501821.15783714
TATCAAC60700.020.4715732
CGTGCGG700.004970808519.64518510
TCAACGC65950.018.7116154
ATCAACG66000.018.697443
AACGCAG66800.018.5146626
TCACGGG750.00741311918.33165241
AGCCGAC750.00741311918.33165241
Produced by FastQC (version 0.11.5)