FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998669
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.3524691364205758No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.3488643384344563No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.21228254807148314No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.19105429326433482No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.14279005356129007No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.1385844559108173No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC11880.11895833354194432No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11620.11635486832974688No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA11360.11375140311754946No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC11200.1121492706792741No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA10320.10333754226875971No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC10250.10263660932701427No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT10250.10263660932701427No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT10120.10133487672091554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28500.037.7274062
GTATCAA91000.037.0947651
GTGGTAT31050.035.3257641
AACGCAG101050.033.8143426
GTCGAGG12200.033.0936788
TATCAAC103650.032.802122
TCAACGC106900.032.0036434
ATCAACG107650.031.8201543
CAACGCA109500.031.243745
ACGCAGA114400.029.9798037
GGCACCG5400.029.906889
CGCAGAG115050.029.7365468
ACAGGTA3650.029.119281
CAGGTAT5150.028.8931121
ATTAGAG6400.028.5541823
GTGACGT300.0068056928.33425338-39
GCAGAGT123250.027.8270939
AAGTACG2150.027.6836321
GGTATCA51400.027.46051
GCTTGTA4250.026.0086561
Produced by FastQC (version 0.11.5)