FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797142
Sequences flagged as poor quality0
Sequence length91
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85811.0764706915455464No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.7296065192901641No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50040.6277426104759253No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.41887141814130985No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.39240185562923546No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.3728319421131994No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.29279601375915454No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.25905045776034885No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.23797516628154078No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.2024733359928344No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.1915593457627374No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.1875450045286787No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA12610.15819013425462464No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.15229407056710095No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.1408782876827466No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.1397492542106676No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT10910.13686394644868793No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.12344099294730425No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.1161649994605729No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21350.065.304451
TGGTATC22950.061.847422
GACAGAC1250.044.207931
GAGTGAC801.1292286E-842.496967
GTATCAA101550.041.0634961
ACGGCTT450.001663325737.775075
TATCAAC113450.037.3089182
TAGTCAC709.2491955E-636.4259645
GGTATCA54000.036.2889141
GACAGGT3400.036.25651
TACCGAG601.5957629E-435.4141358
TCAACGC124900.034.0247884
CCTCGAC250.002789000534.00076730-31
AGTCCCT500.002789454533.9975665
ATCAACG125600.033.7336543
ACAGGGT1404.1836756E-1133.398851
AACGCAG128800.033.0275276
CAACGCA128950.033.0220645
GTCGAGG20000.031.022788
AGTGACC1101.8620631E-730.9068768
Produced by FastQC (version 0.11.5)