FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764355
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.3609579318510378No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.24360408448953694No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16660.217961549280112No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13320.17426457601507153No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.16746145442889757No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9690.12677355417312636No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9410.12311033485749423No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8920.116699701055138No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG8150.10662584793714963No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA7850.10270097009897235No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA7670.10034604339606595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8950.052.70522
GTGGTAT9150.051.098761
GGTATCA17400.041.5277521
GTATCAA33500.039.967271
TATCAAC38600.035.0100982
ATCAACG40700.033.4125063
CAACGCA40600.033.285465
TCAACGC40650.033.2445224
AACGCAG41200.032.8007246
ACGCAGA45650.029.603287
CGCAGAG46300.029.2794678
AGCGTCA1652.8012437E-1028.331023
GCAGAGT48800.027.7794959
CAGAGTA32100.025.02132210-11
CGAGACT1152.724026E-422.1721024
CAGCGTC2357.0940587E-1021.7003562
GAGTACG36600.021.596612-13
AGAGTAC31650.020.87902310-11
GCGTCAG2058.659663E-820.7300174
TATATAG1050.004187369720.2364442
Produced by FastQC (version 0.11.5)