FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764355
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36310.4750410476807243No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11020.144173845922379No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8880.11617638401004769No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8260.10806496981114797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6900.031.4944251
TGGTATC6900.031.0835532
GTATCAA32600.028.2672061
GGTATCA15400.027.5077921
TATCAAC37950.024.4900722
CAGAGTA38700.023.44858210
AGAGTAC38600.023.29560711
ATCAACG39850.023.248853
AACGCAG40600.023.0902296
TCAACGC40050.023.0641084
CAACGCA40950.022.8898815
ACGCAGA41550.022.5637687
CGGGATT2200.022.50195517
CGCAGAG41850.022.2049378
GTCTTAA1003.6037927E-622.0062331
GCAGAGT44650.020.8767729
AGTACGG43550.020.5241713
GAGTACG44250.020.26032312
CCGTTAA1251.1213942E-619.8017216
TCTAGAC1401.533408E-719.6368523
Produced by FastQC (version 0.11.5)