FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746569
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.4958684327905391No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.34116069646610026No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.2153853160257123No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10100.13528555297634914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10900.044.4640851
GTATCAA35350.042.9348341
TGGTATC11400.041.7540552
TATCAAC39250.039.0888632
TCAACGC42100.036.5412064
ATCAACG42400.036.2826653
CAACGCA42450.036.2399255
AACGCAG42700.035.9282236
TTAGTAC500.002789386533.9974563
ACGCAGA48050.032.0163357
CGCAGAG48700.031.589018
GGTATCA19100.030.716851
GCAGAGT51550.029.9250139
GTATTAG2900.024.9219441
CAGAGTA30150.024.45505110-11
TAGTACA1251.7507E-523.798224
TACACGG950.002342450422.3682442
TAGTACT1754.3445652E-721.8555074
AGAGTAC28850.021.72714210-11
GAGTACG33600.021.69108412-13
Produced by FastQC (version 0.11.5)