FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746569
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27530.36875359142959324No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC303.491761E-545.8294833
GTATCAA29500.028.458411
ATCGATA400.00786947527.50137522
AACGCAG32750.026.1070616
AGAGTAC30050.026.07933811
CAGAGTA30150.025.89990210
TATCAAC34400.025.1080462
ACGCAGA34450.024.818767
CAACGCA35150.024.3245035
TCAACGC34850.024.2972354
CGCAGAG35250.024.2554978
ATCAACG35100.024.2073673
GCAGAGT37050.023.005979
AGTACGG34600.022.57186913
GAGTACG34900.022.29755212
AGGTCGC752.8703813E-422.001140
GTGGTAT8700.021.8304461
TGGTATC8750.021.3767932
ACGGGCT2450.020.2050916
GGTATCA14800.020.0860631
Produced by FastQC (version 0.11.5)