FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132803
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.22854812354840162No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.19879890854808824No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.132768009971725No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.12155688147012322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19250.048.5687032
GTGGTAT21050.045.0351451
GTATCAA59750.036.9968031
TATCAAC66800.033.3364562
TCAACGC69850.031.9416584
AACGCAG69850.031.8808176
CAACGCA70550.031.6247335
ATCAACG70700.031.4374183
GGTATCA33350.029.1812733
ACGCAGA79100.028.2063837
CGCAGAG79500.028.066948
CGTCAGA2450.027.7535465
GCAGAGT86750.025.9662539
GTCGAGG8100.025.7107058
GGTACGG1754.3478758E-721.8559173
CAGAGTA51300.021.04356210-11
CTCCGGT2651.4006218E-1020.8478897
TCCGGTC2551.9626896E-920.0006438
AGAGTAC49050.019.88596310-11
TCGAGGT10200.019.5839639
Produced by FastQC (version 0.11.5)