FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123858
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30160.268361305431825No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28660.2550144235303748No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.17164090125264936No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.1439683661103093No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA14260.12688435727645306No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC13600.12101172923981499No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13030.11593991411726393No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT12400.11033422371865484No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT12280.10926647316653884No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12100.10766484733836482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19600.048.80741
TGGTATC19400.048.1954732
GTATCAA66000.036.203591
GGTATCA39200.033.4059521
GTCGAGG8700.032.729618
TATCAAC75000.032.1863982
TCAACGC78800.030.4185264
ATCAACG80050.029.9966283
AACGCAG80350.029.9375236
CAACGCA80800.029.7707885
CGTCAGA2650.028.8676835
GTCCGTT905.141266E-528.3444441
CGCAGAG90450.026.6415678
ACGCAGA91000.026.3871427
TCGAGGT10450.025.6229349
GCAGAGT97200.024.8800149
AGCGTCA3200.023.9060523
AGTCGAG16000.023.3748057
CAGAGTA58200.023.03992310-11
GCAGCGT3750.020.4081
Produced by FastQC (version 0.11.5)