FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259362
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA3940.15191122832180506No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT3300.12723529275684178No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA3290.12684973126363924No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC3010.11605400945396782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.051.563161
TTGAACG250.007789973650.999193
GGTATCA4100.050.7918781
TATCAAC12400.043.5275342
TCAACGC12600.042.836624
ATCAACG12650.042.667313
CAACGCA12800.042.1672975
AACGCAG12950.041.6788756
ACGCAGA13350.040.430077
CGCAGAG13650.039.5414968
GTACTAT450.001660802237.7771766
TGGTATC2050.037.316482
GCAGAGT15250.035.6715629
GTATTAT601.5922774E-435.4161031
GTGGTAT2200.034.772181
GATCGAC500.00278524133.9994629
TGAACCG550.00444206630.9086025
GTACAAG1251.6611011E-830.5995161
TTCTAGT703.9352715E-430.3566592
ATACAGT600.006796539328.3328846
Produced by FastQC (version 0.11.5)