FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259362
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT3620.13957326053932342No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT2750.10602941063070148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.10448716465789129No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT2690.1037160416714862No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT2630.10140267271227088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1550.037.244612
GTGGTAT1500.034.8476331
CTTACAC501.8214836E-532.9817243
CCCGAGT350.00408947531.43540635
TGTAACG350.004104951431.4111654
GGTATCA4550.030.2321831
GCCGACG553.475365E-530.00652555
GTATCAA9250.027.9574171
CTCGTCT400.007868540527.4953713
CTATCGG400.007868540527.4953712
TGCCGAC1309.822543E-1125.39013755
AGAGTCG651.08987806E-425.3705565
TACGGGC1750.025.13862615
GTATGTA550.001221066325.0102631
ACTATCG550.001225194924.99579211
GCAGTAC550.001227953624.9861553
TATCAAC10650.024.0054112
TTACACA952.315628E-623.1450674
ACGGGAG852.5599235E-522.64761516
CAACGCA11300.022.6202075
Produced by FastQC (version 0.11.5)