FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117352
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57540.5149675303753876No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41770.37383027013868503No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.2466545904961015No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT18480.16539103165340913No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.10327989747187995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15500.052.921142
GTGGTAT15700.051.4371571
GTATCAA56550.040.1357731
GGTATCA29600.038.6263431
TATCAAC62900.036.1498682
TCAACGC66300.034.1037144
ATCAACG66950.033.709133
CAACGCA68450.033.0946165
AACGCAG70200.032.2696086
TATACCG1152.74802E-729.5662125
ACGCAGA77300.029.303027
CGCAGAG77550.029.2085578
GCAGAGT81650.027.743119
CAGAGTA45850.026.741810-11
GAGTACG50600.023.97947912-13
GACAGGT5750.022.1756521
AGAGTAC44050.022.04572710-11
TACCGAT1353.1749674E-522.0358097
GCTAGAC1553.7073278E-621.9362223
GTACGGG54950.021.84916714-15
Produced by FastQC (version 0.11.5)