FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812712
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15960.19637952927974486No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA11760.14470070578507516No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8460.1040959158964061No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8260.10163501953951708No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8200.10089675063245038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGGT150.005777905755.01225716
AAGGTCG350.00410311331.42202830
GTGGTAT22000.031.2319551
TGGTATC22500.030.5303972
GGGACCG453.8143146E-430.5285156
GCTTGTA4050.029.1806721
TTGTACG952.5174813E-928.9217553
AGAGTAC50650.025.35946811
GACAGGT2850.025.0732331
GTATCAA73050.025.0573921
CAGAGTA51050.024.99913210
CTTGTAT2500.023.080982
CGGGCCG600.002033111422.92036217
TATCAAC80150.022.7978082
AACGCAG80950.022.6389986
GCTTAAG1103.3028118E-722.487011
CTGCTTA2250.022.00219318
ATCAACG85650.021.4287743
TCAACGC85300.021.4200674
CAACGCA86150.021.240625
Produced by FastQC (version 0.11.5)