FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433333
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190211.3270468202434467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10800.032.0817642
GTGGTAT11350.031.5160581
GTATCAA48700.029.5499761
TATCAAC54700.026.2418612
GGTATCA23350.025.6894021
ATCAACG56700.024.9688913
TCAACGC57000.024.9321944
CAACGCA57450.024.881315
AGAGTAC54450.024.24293111
CAGAGTA55950.023.93371210
AACGCAG59700.023.8531466
CGAACTA701.8010949E-423.571124
CGCAGAG61000.023.34488
ACGCAGA61650.023.0094817
AGTACGG58950.022.11319513
ACGGGAT10700.022.1048116
GCAGAGT64950.021.9704559
GAGTACG61400.021.18603512
CGGGATA2350.021.0657417
GATCGAC3350.020.52353520
Produced by FastQC (version 0.11.5)