FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185475
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56770.4788797739302811No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42830.3612897783588857No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.27448912883021576No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.1965456884371244No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.18667622682890825No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15960.13462957886079419No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.12163900546194563No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.1060334465087834No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA12460.10510554840886564No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.10265927159999157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19750.062.4028972
GTGGTAT19750.061.5655331
GTATCAA80050.041.5321241
TATCAAC91000.036.800922
TCAACGC97350.034.269484
GGTATCA40050.034.1815761
ATCAACG98700.033.8868683
CAACGCA99550.033.469465
AACGCAG103400.032.182156
GTATAGG2200.030.9197561
CGCAGAG116450.028.6121448
GACAGGT4350.028.3431071
ACGCAGA117900.028.224217
GCAGAGT120700.027.7455149
GTCGAGG14750.025.6431798
CAGAGTA60200.024.42606410-11
GTGCGCG900.001711442723.610299
AGTCGAG22250.023.4935677
AGAGTAC56500.022.60319710-11
TCGAGGT15900.022.1847659
Produced by FastQC (version 0.11.5)