FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185475
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32510.27423606571205633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23550.043.5431251
TGGTATC23600.043.0868572
GACAGGT3850.028.5628131
GTATCAA98700.027.9373661
AGAGTAC58500.027.3622711
CAGAGTA61300.026.20217910
CGGGATA4000.025.44056717
TATCAAC109750.025.14112
GGTATCA47200.024.8124311
ATCAACG117150.023.4327553
TCAACGC116900.023.4123464
GAGTACG69100.023.12507212
CAACGCA118800.023.1072985
AGTACGG68900.022.95269213
AACGCAG121200.022.629926
ACGGGAT16550.021.27144416
TACGGGC8950.021.20367215
CCACGCG650.003244685221.1483630
ACGGGCA4950.021.11365516
GTACGGG76550.020.44335214
Produced by FastQC (version 0.11.5)