FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931299
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53880.2789832128531108No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.27566938107460315No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.1868172665133674No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.15171136110980227No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.1053177162106955No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.1001916326783165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28350.052.467682
GTGGTAT29500.050.874281
GTATCAA104650.038.1480141
TATCAAC119000.033.999062
GGTATCA59950.032.693191
TCAACGC123750.032.625364
ATCAACG125400.032.1621863
CAACGCA127700.031.6494755
AACGCAG128900.031.3878046
GTCGAGG16600.028.4186238
CGCAGAG144900.028.0693058
ACGCAGA144900.027.980597
GCAGAGT154000.026.4389469
AGCGTCA5200.024.518553
TCGAGGT19700.024.378769
GCAGCGT5450.023.4029371
CAGAGTA87900.022.50765210-11
GCGTCAG5850.021.7942664
GCTTGTA8100.021.5201071
AGTCGAG27000.021.4068137
Produced by FastQC (version 0.11.5)