FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931299
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32970.17071411521468194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC35500.041.8927272
GTGGTAT36700.040.7698171
GTATCAA126450.025.1881121
GCGGTAC952.3186421E-623.156444
AGAGTAC89400.023.0719711
TATCAAC139100.022.9047742
CAGAGTA92150.022.68188710
GGTATCA69100.022.5157053
TCAACGC147350.021.4906484
ATCAACG148100.021.3818173
CAACGCA149900.021.0883885
AACGCAG150050.021.0495346
CGATCGT1151.2100612E-519.13366147
GTCGAGG16650.018.8251768
ACGCAGA168650.018.7457897
CGCAGAG169500.018.5730928
GAGTACG111250.018.4915712
AGTACGG110900.018.47552913
ACGGGAT15850.018.21932216
CGAGGTT15750.017.8106810
Produced by FastQC (version 0.11.5)