FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687106
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24120.35103754005932125No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.311451217133892No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.20899249897395744No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.16867848628886956No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.1325850742098017No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.10915346394879392No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA6960.10129441454448077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13200.049.2598462
GTGGTAT13750.047.0077361
GTATCAA47300.042.1638371
TATCAAC52950.038.5257262
AGCGTCA1500.036.83223
TCAACGC57400.035.4649354
AACGCAG57400.035.3168566
ATCAACG58150.035.08063
CAACGCA58750.034.6499945
CGTCAGA1700.032.4995
ACGCAGA64650.031.3563427
CGCAGAG65600.030.8397088
GGTATCA23100.030.5579741
GCAGAGT68200.029.6016839
GCGTCAG1951.8189894E-1228.332464
GCAGCGT2053.6379788E-1226.9660851
GTCGAGG7600.025.7247648
CAGCGTC2251.6370905E-1124.55482
GTACGGA1401.5226778E-624.2991121
CAGAGTA36900.023.95938710-11
Produced by FastQC (version 0.11.5)