FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018768
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.32912301917610287No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.2705228275721263No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.18581266784979505No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.14252508912725959No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.1308443139164167No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.1006117192530586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14250.052.512881
TGGTATC15300.047.7740252
GTATCAA50500.041.5072141
TATCAAC59100.035.8812752
TCAACGC62150.033.9836544
ATCAACG63000.033.5251463
CAACGCA64200.032.96475
AACGCAG65400.032.6847426
GGTATCA26850.032.4621731
ACGCAGA73700.029.0038287
CGCAGAG73300.028.9881748
GCAGAGT79850.026.6635289
CAGAGTA40650.023.78466410-11
GACAGGT6150.023.5056251
GTCGAGG9050.023.478828
AGAGTAC38600.022.40542810-11
TCGAGGT9750.020.9214349
GAGTACG46600.020.42858312-13
ACGGGTC1050.004188235420.2365063
AGTCGAG13450.019.90557
Produced by FastQC (version 0.11.5)