FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018768
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25460.24990969484710943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17600.041.860531
TGGTATC17600.041.0654342
TTACGTC400.00786335327.50670836
GTATCAA62600.025.7339151
AGAGTAC38400.023.84995811
CAGAGTA39750.023.24752610
TATCAAC69950.022.8648622
GGTATCA33350.022.7507551
TCAACGC73300.021.5949334
ATCAACG73900.021.531163
CAACGCA74300.021.3042875
CGGGATA2100.020.95337517
AACGCAG76450.020.886936
AGTACGG45300.020.27789513
GAGTACG46300.019.78052512
ACGGGAT9600.018.90807516
ACGCAGA86500.018.397547
GATCGAC2700.018.32969920
GTCGTAT750.007426106418.3269981
ACAGACG1805.809852E-918.3269981
Produced by FastQC (version 0.11.5)