FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165325
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.3239439641301783No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.2438804625319117No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT26930.23109432990796558No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.16690622787634352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10900.051.4793781
TGGTATC11350.049.055392
GTATCAA48000.040.6496961
GGTATCA25300.039.989161
TATCAAC53550.036.4304472
TAACCGA601.5958652E-435.415456
ATCAACG58300.033.3865053
TCAACGC58150.033.2533724
CAACGCA58800.033.030335
AACGCAG62400.031.397166
CGCAGAG66350.029.269288
ACGCAGA67950.028.8302367
GCAGAGT72900.026.639469
CAGAGTA47150.023.838610-11
AGAGTAC44650.020.89054710-11
GAGTACG54150.020.83544712-13
AGTTCCG1254.7845786E-420.3992985
CGACTCT1507.147906E-519.8326535
GTACGGG57400.019.54469114-15
ACGGGAT10050.019.450416-17
Produced by FastQC (version 0.11.5)