FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165325
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55640.4774633685881621No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9700.036.0178381
TGGTATC10250.034.334392
GTATCAA50350.028.4658321
GGTATCA23550.027.801721
CGACGCG400.00787284727.50023514
TATCAAC56100.025.7790012
AGAGTAC54050.024.92655211
TCAACGC58050.024.6677534
CAGAGTA55300.024.46150410
CAACGCA58850.024.3334675
ATCAACG59200.024.1906413
AACGCAG60450.023.9642776
TCGACGT1052.0966581E-723.57365618
CGCAGAG61400.023.5049638
ACGCAGA63100.023.0014137
CCGAATT1950.022.56429714
GCAGAGT66000.021.8695559
AGTACGG61700.021.34853613
GAGTACG64550.020.61808212
CGAATTA2150.020.46704915
Produced by FastQC (version 0.11.5)