FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096084
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.23930647651092435No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.22917951543859777No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC16080.1467040847234336No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.13694205918524494No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA14340.13082938898843519No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.12827484024946992No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC13690.1248991865586944No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT12230.11157903956266126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23150.039.1018072
GTGGTAT24550.037.2436641
GTATCAA71300.035.6082231
AACGCAG76800.033.588896
TCAACGC83200.031.2094464
GCTTGTA5500.030.928781
ATCAACG84500.030.8298873
CAACGCA84250.030.71965
TATCAAC87000.029.8462732
ACGCAGA86550.029.8050467
CGCAGAG86850.029.7020938
TTGTACG1601.9463187E-1029.2173523
GCAGAGT93050.027.7230199
GGTATCA42500.027.7176421
ATTAGAG7750.025.2246023
GGCACCG7450.025.0994729
GTAACGC850.001226860124.9988063
GTCGAGG8900.024.8302738
CAGAGTA57600.024.08956110-11
GTATTAG12450.022.8860531
Produced by FastQC (version 0.11.5)