FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096084
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16620.15163071443429518No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC15340.13995277734188255No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT13980.12754496918119415No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG13500.12316574277153941No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG11770.10738228092007547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23150.030.2716462
GTGGTAT24450.028.9053781
GTATCAA81600.027.1624721
AGAGTAC61500.025.26720811
CAGAGTA61950.025.12806310
CGGGTCG801.6066739E-524.06533617
AACGCAG91100.023.8004766
TATCAAC94300.023.4310192
CGGGATA2350.023.40701517
TCAACGC95600.022.7078694
ATCAACG96550.022.6552053
GACAGGT4400.022.499571
CAACGCA96850.022.4431635
GACAGGC1559.0039975E-1021.2899151
ACGCAGA101850.021.2344367
CGCAGAG102350.021.0856638
GCTTGTA4450.020.392871
GGTATCA41800.020.0654071
GCAGAGT108350.020.0268299
GCGGGAC700.00499750219.6281413
Produced by FastQC (version 0.11.5)