FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476627
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39310.2662148260867504No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG16690.11302786688852365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35100.041.5335581
TGGTATC34800.041.4725072
GTATCAA130300.026.4718021
AGAGTAC79300.025.31666211
CAGAGTA81300.024.65836510
ACGGGGC5050.023.96172516
TATCAAC145650.023.6305642
GGTATCA66600.023.0870231
GTATAGC1605.4569682E-1122.3487741
AGTACGG89350.022.25361413
ATCAACG154850.022.1008473
TCAACGC154900.022.0582224
CGCGTAC752.8721173E-422.00121926
GAGTACG90700.021.95270312
TACGGGG36850.021.86688215
GACAGGT5300.021.7973541
CAACGCA158200.021.6849755
AACGCAG161500.021.3432776
TCGCGTA650.003239140421.15501825
CCGGTCG1055.5169203E-620.95354314
Produced by FastQC (version 0.11.5)