FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344860
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.24032241274184676No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.20834882441295005No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.1427657897476317No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.12670463840102317No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA15740.11703820472019391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19700.047.8923532
GTGGTAT21200.043.3154341
GTATCAA65000.037.7387281
GGTATCA35950.033.4666521
TATCAAC76150.032.760282
TCAACGC79500.031.2194444
ATCAACG81500.030.4533233
CAACGCA81400.030.4385225
AACGCAG82200.030.1939856
CGCAGAG90800.027.28748
ACGCAGA91100.027.2441887
GCAGAGT98350.025.2790629
GTCGAGG9800.023.8515198
GCTTGTA7050.022.3119411
CAGAGTA60900.022.22652410-11
ATACCGT1353.1747557E-522.0365546
TACCGTC1000.003156337421.2495357
AGTACGG76450.019.734692
AGAGTAC58500.019.57863210-11
AGTCGAG16800.019.478747
Produced by FastQC (version 0.11.5)