FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344860
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20830.15488601043974837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23250.039.3881381
TGGTATC23300.038.5696032
GTATCAA84500.026.9149111
TATCAAC94950.023.9656222
AGAGTAC66350.023.79201911
CAGAGTA68050.023.47880610
TCAACGC100450.022.6235334
ATCAACG101200.022.4287153
AACGCAG102050.022.3235136
CAACGCA102200.022.2353155
GGTATCA42550.022.0221483
GCTTGTA3800.020.9873961
ACGCAGA114350.019.9019667
CGCAGAG114600.019.8150028
ACGGGAT12450.018.99789816
GCAGAGT120200.018.9639849
CGGGATC5400.018.84449217
AGTACGG83550.018.6643613
GAGTACG85250.018.38826812
CGTTAAC909.802834E-418.3372339
Produced by FastQC (version 0.11.5)