FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767352
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.41662757117984967No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.38417831712173817No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.2314452819566509No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.20837894473461982No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.16159467884360762No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.15351494490142722No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11650.15182080713935717No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT10150.13227306373085626No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10040.13083956254756618No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10010.13044860767939614No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC8980.11702582387222553No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA8800.11468009466320542No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG8500.11077054598150522No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8270.10777322532553509No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA8070.10516685953773496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGC250.007796879450.9973378
TGGTATC25200.037.1012422
GTATCAA73150.035.3970761
GTGGTAT26100.035.1866261
ACAGGTA3600.033.0689131
GACAGGT2750.032.4676631
AACGCAG80300.032.28356
TATCAAC85550.030.6500682
GTCGAGG10850.029.7680368
TCAACGC87750.029.6394824
ATCAACG88000.029.5552773
CAACGCA90200.028.8344175
GGCACCG3950.027.9750489
ACGCAGA93000.027.9662847
CGCAGAG93350.027.8159058
GGTATCA43050.026.7645871
GTACGGC808.5885363E-426.5732361
GCAGAGT100200.025.9160089
GGGATCC1001.05584666E-425.4986695
GGATCCA850.001226555924.9986956
Produced by FastQC (version 0.11.5)