FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090101
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54080.25874347698986794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32000.041.7554971
TGGTATC33950.039.2545742
GTATCAA120900.026.3108251
AGAGTAC77050.024.6315111
TATCAAC133100.023.7827872
CAGAGTA80400.023.67248310
GGTATCA63200.022.5775931
CGGGATA4500.022.00362217
ATCAACG143100.021.9287973
TCAACGC143500.021.8479984
CAACGCA146600.021.442235
AACGCAG149000.021.2090236
AGTACGG89200.020.96807913
TACGGGG39050.020.56725715
GAGTACG91900.020.56154812
ACGGGGC7350.019.83367216
ACGGGAT17300.019.23773216
GACAGGT10250.019.0441631
ACGCAGA167200.018.868877
GTACGGG99700.018.64945414
Produced by FastQC (version 0.11.5)