FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211027
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA21360.17637922193311956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18040.14896447395475082No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT15630.12906400930780237No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT15550.1284034129709742No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC15060.12435726040790171No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT14280.11791644612382712No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA13210.10908097011875044No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT12630.10429164667674626No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12230.10098866499260546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29000.034.200742
GTGGTAT30850.032.4343831
GTATCAA105450.025.8076271
GGGCTCC651.0889044E-425.38497718
CGAGCCC550.001223833125.00965948
AGACGGT701.8060816E-423.56105431
AGAGTAC78550.023.53064211
CAGAGTA79600.023.32391210
TATCAAC118850.023.0011832
GCCACGA600.002032001522.92362626
CACGGCT600.002046697522.895234
ACAGACG1605.638867E-1122.3348391
AACGCAG123000.022.1385216
GACAGGT4000.021.9912261
GCTTGTA5750.021.9912241
TCAACGC125050.021.6191044
ATCAACG125800.021.5557333
TTCCACG904.0005078E-521.38565843
CAACGCA127650.021.2218065
GGTATCA55550.021.1301841
Produced by FastQC (version 0.11.5)