FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741249
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35640.4808100921552676No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.45639184673436317No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.2950425565498233No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA17580.23716726767928184No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.23595310077989987No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC17490.23595310077989987No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA16220.21881985675528737No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15550.20978105872655478No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13950.18819586940420832No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13650.18414864640626835No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13480.181855220040769No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.18064105314138704No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.17510984837753577No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA12920.17430040377794778No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC12410.16742012468144982No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC12220.16485688344942118No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11820.15946058611883457No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG10600.1430018792605454No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA10350.13962919342892874No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGA10320.13922447112913475No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG10110.13639141503057678No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG9870.1331536366322248No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT9460.12762243186837352No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC9320.1257337278026682No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC9030.1218214122379929No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT8850.11939307843922892No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA8600.1160203926076123No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.11386187367537763No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT8380.11305242907578965No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT8190.110489187843761No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA8170.11021937297723168No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG8170.11021937297723168No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT7740.10441835334685107No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG7600.10252964928114575No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA7440.1003711303489111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22100.048.2682722
GTGGTAT23650.044.5777441
CGTCAGA1350.037.7770275
GTATCAA76750.037.7749181
GCTTGTA3650.036.104921
GCTTCTC601.5926409E-435.425521
GGTATCA41850.034.5367131
TATCAAC86050.033.8807952
AACGCAG91000.032.0378276
TCAACGC91750.031.7759364
CAACGCA94600.030.8635545
ATCAACG94800.030.8432713
GTCGAGG10450.029.690418
ACGCAGA102650.028.403697
GTCCGAC600.006802466728.334688
CGCAGAG103300.028.1838198
GACAGGT2750.027.8251381
TAGGCCA2750.027.817635
TCGAGGT11450.027.0973599
GCAGAGT108550.026.9773339
Produced by FastQC (version 0.11.5)