FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323335
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.46617069751801316No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40720.3077074210233992No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT30200.2282112994819906No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.21370250163412893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15150.056.1072041
TGGTATC15900.052.6527632
GTATCAA54100.040.931
TATCAAC60450.036.7669372
TCAACGC64350.034.47264
ATCAACG64650.034.3783653
CAACGCA66150.033.5988125
GGTATCA28600.033.2876631
AACGCAG67450.033.077256
ACGCAGA76800.029.1056027
CGCAGAG77350.028.8986478
GCAGAGT82400.027.436999
CAGAGTA46100.024.33632910-11
GCTTACG1102.0157512E-423.1824781
AGAGTAC43300.022.42592410-11
TCTACAC4100.020.7299483
GAGTACG56050.020.01614212-13
TAGTACT1708.258858E-619.99834
CTTACGC1306.2347215E-419.613722
AGTACGG50900.019.03571112-13
Produced by FastQC (version 0.11.5)