FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643071
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.22470302657093852No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.209463651758515No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.1408864651026092No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.12347003674555376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC302.2551676E-456.6631977
GTGGTAT11400.041.7615661
TGGTATC11750.040.8698352
GTATCAA36350.034.263111
GTACGGA751.4809273E-534.0058481
CCTTATA1008.0606696E-833.9979172
GTGCGCA500.002788915533.9979179
TATCAAC39500.031.846152
GTCGACA703.942743E-430.3552828
ATCAACG41550.030.0703623
AACGCAG41300.030.0465856
TCAACGC42000.029.6469944
GGTATCA21350.029.4664251
CAACGCA42800.029.0928445
GTCTAGG1106.4817687E-627.0501061
ACGCAGA46400.026.7440517
GTATAGA1606.2245817E-926.567071
CGTAGCA408.609226E-426.56396958-59
CGGGATT2105.456968E-1226.307914
CGCAGAG47450.026.0626818
Produced by FastQC (version 0.11.5)