FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643071
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9690.1506832060534529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13050.036.2220951
TGGTATC13600.034.7518272
GTATCAA43000.024.2229161
TCTACGA600.002043968322.8984892
GCTTGTA2200.022.4856511
TATCAAC45950.022.3054732
AGAGTAC31700.022.04121811
CAGAGTA32500.021.75258610
ATCAACG49400.020.8017063
TCAACGC49450.020.6695464
AACGCAG49650.020.6416266
GGTATCA23750.020.5973821
CAACGCA50900.020.1886885
TGTCGCC700.0049651919.64866810
GTGTAAG2105.456968E-1219.630331
TAGTTCG856.7082606E-419.3993857
CGGGTCA1301.6311842E-619.04112817
ACGCAGA55350.018.5699027
CGCAGAG56150.018.407518
ACACGCC750.00741241118.3316233
Produced by FastQC (version 0.11.5)