FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170822
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69340.3194181743136932No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.2987808304872532No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.19573230785389129No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT35660.1642695716184929No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA35180.16205842763708864No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA30770.14174354230793682No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC30100.13865715383389335No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC29890.13768977834202895No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT27610.12718684443035863No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA26970.12423865245515292No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.12124439498033464No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT25390.11696030351636384No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG25050.11539407652953582No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24700.1137817840430952No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA23940.11028080607253841No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA23870.1099583475752503No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC22640.10429229112290184No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT21970.10120590264885836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC47900.040.9906732
GTGGTAT49300.038.9789541
GTATCAA165900.038.773161
AACGCAG191900.033.9061746
TATCAAC197600.033.1001742
TCAACGC198000.032.8830534
ATCAACG198850.032.785243
GACAGGT9100.032.7035831
CAACGCA203750.032.0176435
ACGCAGA208850.031.195097
CGCAGAG211000.030.8779378
GGTATCA92200.029.6957021
CAGACGT1302.4505425E-829.4223732
GCAGAGT226850.028.7767019
ATTAGAG13600.027.8118363
GACAGGG6300.026.9934331
GGCACCG13950.026.5053549
GTCGAGG18550.025.6603578
GCTTGTC5500.025.5087931
AGACAGG7100.024.5507141
Produced by FastQC (version 0.11.5)