FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524414
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.5057681181096474No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58800.3857219889085248No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37550.24632416128427057No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.1045647704626171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19750.056.8134422
GTGGTAT21250.052.818671
GTATCAA72050.039.299111
TATCAAC80000.035.5426982
GGTATCA37300.033.738471
ATCAACG86100.033.120043
TCAACGC86150.033.100824
CAACGCA88500.032.173855
AACGCAG92600.030.7493066
CGCAGAG102400.027.8876788
ACGCAGA103450.027.6037187
GCAGAGT111800.025.5817669
CAGAGTA61650.023.74764410-11
AGAGTAC57900.021.43229310-11
GAGTACG67250.021.39203512-13
GTACGGG71200.019.99601614-15
AGTACGG64000.019.25767112-13
AGCGTCA3007.403287E-1018.4159283
GCTTGTA4850.018.408571
GCGTCAG3201.7498678E-917.2649334
Produced by FastQC (version 0.11.5)