FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003576
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT66220.6598404106913677No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.38123669756949147No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.2772087016827824No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.1636149130708586No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT13410.1336221671303419Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT11180.11140162777906208Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12300.052.1864242
GTGGTAT12550.051.4958421
GTATCAA41250.038.755751
TATCAAC45500.034.7549932
GGTATCA23150.034.3449481
ATCAACG48850.032.453763
CAACGCA49700.031.9842365
TCAACGC49600.031.9630244
AACGCAG51350.030.9565056
ACGCAGA56650.028.131137
CGCAGAG58550.027.3634158
GCAGAGT61350.025.9760179
CAGAGTA35850.024.41933810-11
GTACTAA1404.187093E-521.2589741
GAGTACG41700.020.99360512-13
AGAGTAC34600.020.20438410-11
ACTCGGT2602.4865585E-919.6236691
GTACGGG44600.019.4379814-15
AAGGGGG17950.018.1132468-69
AGGGGGG18400.017.90257370-71
Produced by FastQC (version 0.11.5)